炎症
软骨细胞
骨关节炎
MAPK/ERK通路
体内
一氧化氮
细胞凋亡
一氧化氮合酶
阿格里坎
NF-κB
化学
阿达姆斯
激酶
癌症研究
基质金属蛋白酶
血栓反应素
信号转导
药理学
软骨
金属蛋白酶
医学
免疫学
内科学
体外
生物
生物化学
病理
解剖
生物技术
替代医学
关节软骨
作者
Li Teng,Yue Shen,Yuhan QU,Longfei Yang,Yuting YANG,Xi JIAN,Shengli FAN,Lele ZHANG,Qiang Fu
标识
DOI:10.1016/s1875-5364(23)60388-7
摘要
Osteoarthritis is a prevalent global joint disease, which is characterized by inflammatory reaction and cartilage degradation. Cyasterone, a sterone derived from the roots of Cyathula officinalis Kuan, exerts protective effect against several inflammation-related diseases. However, its effect on osteoarthritis remains unclear. The current study was designed to investigate the potential anti-osteoarthritis activity of cyasterone. Primary chondrocytes isolated from rats induced by interleukin (IL)-1β and a rat model stimulated by monosodium iodoacetate (MIA) were used for in vitro and in vivo experiments, respectively. The results of in vitro experiments showed that cyasterone apparently counteracted chondrocyte apoptosis, increased the expression of collagen II and aggrecan, and restrained the production of the inflammatory factors inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), a disintegrin and metalloproteinase with thrombospondin motifs-5 (ADAMTS-5), metalloproteinase-3 (MMP-3), and metalloproteinase-13 (MMP-13) induced by IL-1β in chondrocytes. Furthermore, cyasterone ameliorated the inflammation and degenerative progression of osteoarthritis potentially by regulating the nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. For in vivo experiments, cyasterone significantly alleviated the inflammatory response and cartilage destruction of rats induced by monosodium iodoacetate, where dexamethasone was used as the positive control. Overall, this study laid a theoretical foundation for developing cyasterone as an effective agent for the alleviation of osteoarthritis.
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