菠萝蛋白酶
TBARS公司
化学
食品科学
凝胶电泳
硫代巴比妥酸
色谱法
生物化学
抗氧化剂
酶
脂质过氧化
蛋白酶
作者
Ziqing Ye,Jian Zhang,José M. Lorenzo,Mutian Zhang,Wangang Zhang
摘要
Abstract This study was aimed to assess the effects of bromelain on the eating quality of smoked salted duck. Whole ducks were marinated with different doses of bromelain (300 U/g, 600 U/g, 900 U/g, 1,200 U/g and 1,500 U/g), while the group without bromelain was considered as control (CK). After the production of smoked salted duck was completed, the pH, color, texture, electronic tongue detection, thiobarbituric acid reactive substances (TBARS), sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE), and mass spectrometry analysis were determined. The results showed that, compared to CK, the pH, TBARS and hardness values in 900, 1,200 and 1,500 U/g groups were reduced. The cohesiveness and the springiness were increased while the values of b* were decreased in all bromelain treatments ( p < .05). The SDS‐PAGE and mass spectrometry analysis indicated that myosin and actin were further hydrolyzed into small‐molecule proteins by bromelain. Electronic tongue detection showed that the umami, the saltiness and the richness of smoked salted duck were enhanced, while the bitterness was reduced at the dose of 900 U/g. Thus, bromelain improved the eating quality of smoked salted duck in particular at the level of 900 U/g.
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