醇溶蛋白
化学
无规线圈
变性(裂变材料)
蛋白质聚集
静水压力
粒径
卵清蛋白
蛋白质二级结构
傅里叶变换红外光谱
光谱学
荧光光谱法
生物物理学
粒子(生态学)
结晶学
圆二色性
分析化学(期刊)
化学工程
荧光
食品科学
色谱法
生物化学
面筋
核化学
光学
物理化学
热力学
工程类
地质学
免疫学
物理
海洋学
生物
量子力学
免疫系统
作者
Yidan Zhang,Xuebing Zhang,Zhiheng Zhang,Zhenjia Chen,Jing Xu,Xiaowen Wang
标识
DOI:10.1016/j.lwt.2021.112755
摘要
The main purpose of this study was to investigate the effects of HHP pressure on the structure and physicochemical properties of millet gliadin. The effects of HHP pressure on its physicochemical properties, structure, and conformation were systematically studied by ultraviolet second derivative spectroscopy, fluorescence spectroscopy, Fourier transform infrared spectroscopy, and particle size analysis. HHP pressure treatment changed the spatial conformation of millet gliadin and increased the hydrophilicity of gliadin, but it did not alter the distribution of subunits. HHP pressure treatment changed the surface charge distribution of protein and increased the aggregation degree of protein, leading to the increase in protein particle size. After treatment, the α-helices and random coil ratio of the protein decreased, and some disulfide bonds formed, which made the protein structure more orderly and led to an increase in denaturation temperature. This phenomenon helped improve the limitation that millet gliadin cannot be directly used in the field of food production due to its strong hydrophobicity.
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