Vaginally isolated Lactobacillus strains (n=511) were evaluated for probiotic properties in the female urogenital region. A strain of Lactobacillus plantarum designated LB931 showed several desirable properties and was found to grow and reduce the pH in MRS broth rapidly, to produce substances totally inhibiting growth of common urogenital pathogens, and to remain viable during long-term storage. It was not affected by antimycotics or spermicidals and it adhered to the urogenital surface. Strain LB931 possesses properties required by probiotic bacteria aimed to improve or restore the female urogenital microflora.