The Characteristic and Application of Molecularly Imprinted Polymer: Efficient Sample Preconcentration of Antibiotic Cefathiamidine from Human Plasma and Serum by Solid Phase Extraction

Abstract Polymers imprinted with cefathiamidine were synthesized using 4‐vinylpyridine as an interacting monomer, ethyleneglycol dimethacrylate as a cross‐linker, and methanol and toluene as a porogen. The polymer was used as solid‐phase extraction sorbent. One‐hundred percent of the cefathiamidine was bound to the polymer and the target can be eluted efficiently only by 1 ml eluant. Human plasma and serum samples were preconcentrated by the molecularly imprinted solid‐phase extraction prior to analysis by HPLC. Recoveries of up to 98% were achieved. The molecularly imprinted solid phase extraction column can be used repeatedly.