The Characteristic and Application of Molecularly Imprinted Polymer: Efficient Sample Preconcentration of Antibiotic Cefathiamidine from Human Plasma and Serum by Solid Phase Extraction

Polymers imprinted with cefathiamidine were synthesized using 4‐vinylpyridine as an interacting monomer, ethyleneglycol dimethacrylate as a cross‐linker, and methanol and toluene as a porogen. The polymer was used as solid‐phase extraction sorbent. One‐hundred percent of the cefathiamidine was bound to the polymer and the target can be eluted efficiently only by 1 ml eluant. Human plasma and serum samples were preconcentrated by the molecularly imprinted solid‐phase extraction prior to analysis by HPLC. Recoveries of up to 98% were achieved. The molecularly imprinted solid phase extraction column can be used repeatedly.