Taurine Protects Irinotecan-induced Muscle Dysfunction by Modulating Oxidative Stress and Endoplasmic Reticulum Stress in Human Skeletal Muscle Cells

牛磺酸 内质网 伊立替康 骨骼肌 未折叠蛋白反应 内分泌学 氧化应激 心肌细胞 内科学 生物 药理学 化学 生物化学 医学 癌症 氨基酸 结直肠癌
作者
Chih‐I Chen,YU-CHI CHEN,Yi-Kai Kao,Chia‐Hung Chen,Pinglin Yang,P. C. Chen,Ling‐Chiao Song,Kai Lung Tsai
出处
期刊:Anticancer Research [International Institute of Anticancer Research (IIAR) Conferences 1997. Athens, Greece. Abstracts]
卷期号:44 (12): 5371-5378 被引量:1
标识
DOI:10.21873/anticanres.17364
摘要

Background/Aim: Irinotecan is a key component of standard first-line treatment for metastatic colorectal cancer. However, irinotecan-induced muscle dysfunction is a contributing factor to cancer cachexia. Here, we present the protective effect of taurine, a conditionally essential amino acid with great antioxidant properties, in attenuating muscle dysfunction induced by irinotecan. Materials and Methods: Irinotecan (20 μg/ml) was added to human skeletal muscle cells (HSkMCs) with or without pre-treatment of taurine (5 mM). The effects of taurine and irinotecan on the viability, cytotoxicity, and differentiation ability of HSkMC myoblasts were examined. The intracellular reactive oxygen species (ROS) and endoplasmic reticulum stress (ERS) were also monitored. Results: Irinotecan caused cytotoxicity of HSkMCs, while taurine pretreatment increased cell viability and inhibited adenylate kinase release significantly in both myoblasts and myotubes. During differentiation, taurine increased ROS clearance and preserved the myotube differentiation ability impaired by irinotecan. Irinotecan exposure resulted in the up-regulation of CCAAT/enhancer-binding protein homologous protein (CHOP) and glucose-regulated protein 78 (GRP78). Taurine pretreatment could combat such irinotecan-induced ERS. Conclusion: The current in vitro study provides molecular evidence that taurine plays a beneficial role in protecting against irinotecan-induced muscle dysfunction by modulating oxidative stress and endoplasmic reticulum stress.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
华仔应助Shaangueuropa采纳,获得10
2秒前
rong完成签到 ,获得积分10
4秒前
小李子发布了新的文献求助10
7秒前
7秒前
天天快乐应助JamesTYD采纳,获得10
8秒前
9秒前
万能图书馆应助zlj采纳,获得20
12秒前
青蛙的第二滴口水完成签到,获得积分10
14秒前
LHP完成签到,获得积分10
14秒前
HC发布了新的文献求助10
14秒前
17秒前
小李子完成签到,获得积分10
18秒前
科研通AI5应助可靠的中心采纳,获得10
20秒前
steven发布了新的文献求助50
20秒前
22秒前
22秒前
疯狂的夏天完成签到,获得积分10
25秒前
az完成签到 ,获得积分10
26秒前
宇宙最萌小猫咪完成签到 ,获得积分10
27秒前
甜甜千亦发布了新的文献求助10
28秒前
28秒前
29秒前
jialin完成签到,获得积分10
30秒前
Freya发布了新的文献求助10
31秒前
nieinei发布了新的文献求助10
33秒前
wy发布了新的文献求助10
33秒前
34秒前
35秒前
Jasper应助AA采纳,获得10
35秒前
Wency发布了新的文献求助10
38秒前
陆小果完成签到,获得积分10
38秒前
阿达完成签到 ,获得积分10
39秒前
zlj发布了新的文献求助20
39秒前
麓麓菌完成签到 ,获得积分10
40秒前
lmgj完成签到,获得积分10
41秒前
跳跃的大叔关注了科研通微信公众号
42秒前
42秒前
Jasper应助甜甜千亦采纳,获得10
46秒前
美好斓应助steven采纳,获得200
47秒前
47秒前
高分求助中
Les Mantodea de Guyane Insecta, Polyneoptera 2500
Technologies supporting mass customization of apparel: A pilot project 450
Brain and Heart The Triumphs and Struggles of a Pediatric Neurosurgeon 400
Cybersecurity Blueprint – Transitioning to Tech 400
Mixing the elements of mass customisation 400
Периодизация спортивной тренировки. Общая теория и её практическое применение 310
the MD Anderson Surgical Oncology Manual, Seventh Edition 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3783222
求助须知:如何正确求助?哪些是违规求助? 3328565
关于积分的说明 10236984
捐赠科研通 3043669
什么是DOI,文献DOI怎么找? 1670627
邀请新用户注册赠送积分活动 799792
科研通“疑难数据库(出版商)”最低求助积分说明 759126