Acetyl Bromide Method for Total Lignin Content Determination in Plant Biomass

Lignin is a tough biopolymer that gives plants strength and protection. It is also a major obstacle for converting plant biomass into biofuels because it prevents enzymes from accessing the sugar-rich fibers. To optimize biofuel production, we need to measure the lignin content in plant tissues accurately and efficiently. In this protocol, we describe a simple and reliable method to measure the total lignin content in plant tissues. The method uses acetyl bromide, a chemical that dissolves lignin into soluble derivatives and makes it possible to detect them by their absorbance at 280 nm. The method consists of two steps: first, we obtained destarched cell wall material from the plant samples, and second, we treat the cell wall material with acetyl bromide and measure the absorbance of the lignin solution. This method can capture all types of lignin and works well with different plant tissues.