聚糖
糖组学
唾液酸
衍生化
化学
鞘脂
鞘糖脂
糖脂
神经酰胺
生物化学
神经节苷脂
质谱法
色谱法
糖蛋白
细胞凋亡
作者
Hisatoshi Hanamatsu,Takashi Nishikaze,Yoshihiro Furukawa
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 289-299
被引量:2
标识
DOI:10.1007/978-1-0716-2910-9_21
摘要
Glycosphingolipids (GSLs) are glycolipids with ceramide and carbohydrate head groups that play an important role in numerous biological processes. Previously, we performed GSL-glycan analysis of various cell lines and virus-infected cells using a glycoblotting approach. Recently, we developed several methods for sialic acid linkage-specific chemical modification to distinguish sialylated glycan isomers by mass spectrometry. In this chapter, we describe a method for analyzing GSL-glycans in human serum/plasma using glycoblotting combined with aminolysis-SALSA (sialic acid linkage-specific alkylamidation) and lactone-driven ester-to-amide derivatization (LEAD)-SALSA for comprehensive and detailed structural glycomics.
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