水杨酸
生物生产
苯甲酸
细胞生长
生物化学
细胞信号
转录组
化学
生物
信号转导
基因表达
基因
作者
Liang Fu,Qingcheng Li,Congli Chen,Yueju Zhang,Yinglu Liu,Liang Xu,Yihan Zhou,Chengbin Li,Dandan Zhou,Bruce E. Rittmann
出处
期刊:Chemosphere
[Elsevier]
日期:2021-02-01
卷期号:265: 129084-129084
被引量:15
标识
DOI:10.1016/j.chemosphere.2020.129084
摘要
Cell-to-cell communication regulates microalgae production via signaling molecules (SMs), but few microalgal SM species are known. Here, we document two new microalgae SMs, benzoic acid (BA) and salicylic acid (SA). Initially, crude SMs were extracted from a microalgae culture in which microalgae grew on heterotrophic-enriched phosphorus nutrition. The extracted SMs enhanced Chlorella growth by ∼72%, promoted nutrient uptake, and up-regulated the mitogen-activated protein-kinase signaling cascade. Fourier transform infrared and nuclear magnetic resonance analyses identified the putative SMs was aromatic carboxylic acids. BA and SA were identified using high-resolution mass spectrometry. BA and SA addition increased cell growth by ∼75% and ∼25%; and improved ATP production by ∼35% and ∼20%. Transcriptomic analysis showed that BA and SA were biosynthesized via CoA-dependent, non-oxidative pathway. The SMs upregulated TCA-cycle enzymes, which promoted carbon assimilation and activated DNA-replicating enzyme, so that accelerated cell division. This study identified two new SMs for microalgae cell communication and provides means to identify other SMs.
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