细胞生物学
体内
结蛋白
心肌细胞
胞浆
化学
脂质过氧化
体外
细胞毒性T细胞
心肌
氧化磷酸化
细胞骨架
氧化应激
肌动蛋白
生物化学
生物
内分泌学
细胞
免疫学
酶
生物技术
波形蛋白
免疫组织化学
作者
Ambra Antonioni,Ivan Dimauro,Cristina Fantini,Rosario Barone,Filippo Macaluso,Valentina Di Felice,Daniela Caporossi
标识
DOI:10.1016/j.freeradbiomed.2020.03.013
摘要
The αB-crystallin (HSPB5) protein is modulated in response to a wide variety of stressors generated by multiple physio-pathological conditions, sustained by reactive oxygen species (ROS) production. In cardiac muscle tissue, this protein regulates various cellular processes, such as protein degradation, apoptosis and the stabilization of cytoskeletal elements. In this work, we studied the role of HSPB5 expression, activation and localization in HL-1 murine cardiomyocytes exposed to pro-oxidant and non-cytotoxic H2O2 concentration, as well as in cardiac tissue isolated from mice following an acute, non-damaging endurance exercise. Our results demonstrated that HSPB5 is the most abundant HSP in both cardiac muscle tissue and HL-1 cells when compared to HSPB1 or HSPA1A (≈3–8 fold higher protein concentrations, p < 0.01). The acute exposure of cardiac muscle cells to sustainable level of H2O2 “in vitro” or to aerobic non-damaging exercise “in vivo” determined a fast and specific increase of HSPB5 phosphorylation (from 3 up to 25 fold increase, p < 0.01) correlated to an increase in lipid peroxidation (p < 0.05). In both experimental models, p-HSPB5 likely facilitated both the interaction with β-actin, desmin, and α-Filamin 1, the last one identified as new HSPB5 substrate in cardiac cells, as well as the sub-localization of HSPB5 within the same cellular compartment or the re-localization between compartments (i.e., nucleus and cytosol). Taken together, these data point out the role of “oxidative eustress” induced by physiological conditions in activating the molecular machinery devoted to cardiomyocytes’ protection and candidate HSPB5 as a putative molecular mediator for the health benefits induced in cardiac tissue by exercise training.
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