生物发光
化学
荧光
亲缘关系
钙
生物物理学
罗丹明
生物化学
生物
物理
有机化学
量子力学
作者
Nicole Mertes,Marvin Busch,Magnus-Carsten Huppertz,Christina Nicole Hacker,Jonas Wilhelm,Clara-Marie Gürth,Stefanie F. Kühn,Julien Hiblot,Birgit Koch,Kai Johnsson
摘要
We introduce a family of bright, rhodamine-based calcium indicators with tuneable affinities and colors. The indicators can be specifically localized to different cellular compartments and are compatible with both fluorescence and bioluminescence readouts through conjugation to HaloTag fusion proteins. Importantly, their increase in fluorescence upon localization enables no-wash live-cell imaging, which greatly facilitates their use in biological assays. Applications as fluorescent indicators in rat hippocampal neurons include the detection of single action potentials and of calcium fluxes in the endoplasmic reticulum. Applications as bioluminescent indicators include the recording of the pharmacological modulation of nuclear calcium in high-throughput compatible assays. The versatility and remarkable ease of use of these indicators make them powerful tools for bioimaging and bioassays.
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