Experimental research on intervention effects of“Yishen Baozhen Formula”on renal fibrosis of chronic renal failure rats

Objective To explore the intervention effects ofYishen Baozhen Formulaon renal fibrosis of chronic renal failure(CRF)and its therapeutic effectiveness mechanism.Methods The CRF models were replicated by two-step of 5/6nephrectomy and randomized into sham-operated group,model group,high-doseYishen Baozhen Formulagroup,low-doseYishen Baozhen Formulagroup andNiaoduqinggroup,with the course of 6weeks. The Scr,BUN,urine protein quantity and pathological changes of kidney tissues were observed,and the glomerulosclerosis index(GSI),type Ⅳ collagen content and expressions of CTGF and a-SMA were detected.Results Compared with the model group,the urine protein,Scr,BUN and content of typeⅣcollagen inYishen Baozhen Formulagroups were decreased significantly.No significant difference was found betweenYishen Baozhen Formulagroups andNiaoduqinggroup;the GSI and expressions of CTGF and a-SMA were down-regulated,with significant differences betweenYishen Baozhen Formulagroups andNiaoduqinggroup(P0.05).Conclusion Yishen Baozhen Formulacan retard the progress of renal failure aggravation,interfere with the process of chronic renal failure fibrosis.Its mechanism may be related to down-regulating the expressions of CTGF and a-SMA,inhibiting the transdifferentiation of inherent kidney cells,thus to reduce the synthesis and accumulation of extracellular matrix.