Objective: To image the structure of the lipid bilayer at the nanometer level with AFM. Methods: AFM images the lipid bilayer that was formed by the vesicle fusion method. Results: The fluidity of the lipid bilayer can be investigated with AFM in fluid. The height and topography of the lipid bilayer can be calculated and observed by AFM. Conclusion: AFM is a good tool to image the lipid bilayer.