Objective to find resistant and sensitive osteosarcoma tumor tissue samples,so that to prepare for further looking for differences in protein.Methods Obtain the tissue of patients with osteosarcoma,osteosarcoma cells isolated and cultured in vitro drug sensitivity test cells,so as to identify drug-resistant and sensitive osteosarcoma tissue samples.Results Detection curve of osteosarcoma cells to various chemotherapy drugs and Found resistant and sensitive osteosarcoma tissue samples.Conclusion Osteosarcoma has different sensitivity to various chemotherapeutic drugs,Cisplatin is further appropriate drugs to look for differences in protein research.