Influence factors on microcrystalline cellulose hydrolyzed by cellulase

The main influence factors of cellulase activity were studied,and the hydrolysis conditions of microcrystalline cellulose were optimized.The experimental results showed that the cellulase optimum temperature and pH were 50 ℃ and 4.8,respectively.The cellulase activity was improved with the increase of reaction time and enzyme dilution degree.The mass concentration of reducing sugars was up to 2.398 mg/mL,under the optimized hydrolysis conditions with 50 ℃,pH 4.8,m(microcrystalline cellulose):m(cellulase)=1:0.12,and oscillation reacting 12 h.