Effect of non-ionic surfactant TX-100 on biphenol A(BPA) degradation in the natural surface coatings by Pseudomonas putida was investigated.TX-100 inhibits the biodegradation of BPA in biofilm at low concentrations(1.0 critical micelle concentration,CMC),however,it enhances BPA degradation in the presence of TX-100 at high concentrations(1.0 times of CMC),and the degradation rate of BPA increases gradually with the increase of BPA concentration.The removal of the main components of the natural surface coatings by the selective extraction techniques has an effect on the BPA degradation,leading to a significant decrease in the biodegradation of BPA.