Effects of enriched-copper on Spirodela polyrhiza

The effects of copper on growth,pigment content,chlorophyll a fluorescence and Cu accumulation in tissue of Spirodela polyrhiza were conducted. The results showed that copper could depress the growth adversely on both fresh weight and dry weight basis. And the fresh weight would be negative growth in medium of copper-added at 8 μmol L-1 ,the dry weight at 2 μmol L-1 ;the content of chlorophyll decreased greatly, but not the carotenoid;to chlorophyll a fluorescence,in first 24 h,copper made the Fo and Fm bigger,but not Fv/ Fm,then Fo and Fm reduced to very low,specially Fm.so as to Fv/Fm would be negative value,which demonstrated the photosynthesis apparatus had been damaged;copper content in tissue increased with the concentration of copper-added in medium, so the accumulation of copper happened. Because of biomass growth being very little at high copper concentration addition,so it is only available that using Spirodela polyrhiza to restore the environment polluted by low concentration copper.