OPTIMIZATION OF ANTIOXIDANT ACTIVE COMPONENTS DERIVED FROM CORDYCEPS MILITARIS WITH NEUTRASE USING RESPONSE SURFACE METHODOLOGY

The effect of process conditions on antioxidant activity of Cordyceps militaris hydrolyzed with Neutrase has been investigated using response surface methodology (RSM) in this paper. RSM was including three parts: Fractional factorial design(FFD),steepest ascent design,and central composite design(CCD).The optimum conditions of enzymatic hydrolysis were: 6.5 pH value, 45 ℃ temperature, 7.03 % ratio of enzyme and substrate, 25.41 ratio of water and raw material, 4 h time, the predicted value at stationary point of reducing power(A700) was 0.465.