聚电解质
等温滴定量热法
离子强度
阳离子聚合
化学
动态光散射
高分子化学
滴定法
Zeta电位
牛血清白蛋白
聚合
聚苯乙烯
化学工程
生物物理学
聚合物
色谱法
有机化学
纳米颗粒
物理化学
水溶液
工程类
生物
作者
Siyi Wang,Kaimin Chen,Li Li,Xuhong Guo
出处
期刊:Biomacromolecules
[American Chemical Society]
日期:2013-02-26
卷期号:14 (3): 818-827
被引量:104
摘要
Cationic spherical polyelectrolyte brushes (SPBs) were synthesized by photoemulsion polymerization, consisting of a polystyrene core with a diameter around 80 nm and a poly(2-aminoethylmethacrylate hydrochloride) (PAEMH) shell with a thickness from 10 to 50 nm densely grafted on the core surface. The binding of various proteins onto SPBs was observed by turbidimetric titration, dynamic light scattering (DLS), zeta potential, and isothermal titration calorimetry (ITC). The binding, aggregation, and releasing of proteins by SPB can be tuned by modulating pH. The pH regions of binding for bovine serum albumin (BSA), β-lactoglobulin (BLG), and papain onto SPBs are markedly different and tunable by ionic strength and stoichiometry between protein and SPB. Binding energetics, affinity, and amount of various proteins onto cationic SPBs were determined by ITC. These findings lay the foundation for SPB applications in the protein purification and selective immobilization of different proteins, enzymes, and antibodies.
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