Near-Infrared Light-Inducible CRISPR-Cas12a/Tetrahedral DNA Nanosystem for Spatiotemporal Control of mtDNA Mutation Imaging and Gene Therapy

-cleavage, and generates amplified fluorescence for mtDNA mutation detection with a detection limit of 0.83 pM. In living cells and tumor-bearing mice, the nanosystem enables high-spatiotemporal resolution imaging of mtDNA mutations while inducing DNA self-assembly and CRISPR-based mtDNA editing. These disrupt the mitochondrial membrane potential, increase the level of reactive oxygen species, and promote apoptosis, resulting in effective tumor suppression. This nanosystem enables the controllable imaging of mutant mtDNA and gene modulation, offering new opportunities for spatiotemporal cancer theranostics.