下调和上调
硫酸软骨素
细胞凋亡
化学
骨关节炎
镁
软骨素
钠
实时聚合酶链反应
信使核糖核酸
基因表达
分子生物学
细胞生物学
生物化学
基因
生物
医学
糖胺聚糖
病理
有机化学
替代医学
作者
Sijing Li,Fenbo Ma,Xiangchao Pang,Bin Tang,Lin Li
标识
DOI:10.1016/j.carbpol.2019.02.061
摘要
Magnesium chondroitin sulfate (MgCS) has been fabricated and characterized in this study. We investigated its morphology, composition as well as structure. The results verify that the sodium of sodium chondroitin sulfate (CS) is successfully replaced by magnesium and formed a polysaccharide-metal ion complex. To evaluate the clinical potential of MgCS, cell proliferation and apoptosis test were conducted. The results reveal that MgCS could effectively increase the proliferation and decrease the apoptosis of osteoarthritis (OA) chondrocytes. Moreover, real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to evaluate the gene expression level. RT-qPCR analysis suggests that MgCS could significantly increase the expression of COLII and decrease the expression of IL-1β and iNOS in OA chondrocytes. Furthermore, significant upregulation of Bcl-2 mRNA expression and downregulation of the expression of apoptosis related gene p53 were observed. Thus, it is indicated that MgCS should have great potentials in OA treatment.
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