厚壁菌
粪便
代谢组学
肠道菌群
没食子酸
新陈代谢
微生物群
微生物学
益生菌
生物
代谢组
脂肪酸
生物化学
乳酸
结肠炎
细菌
食品科学
碳水化合物代谢
短链脂肪酸
蛋白质细菌
发酵
16S核糖体RNA
生物信息学
免疫学
丁酸盐
基因
遗传学
抗氧化剂
作者
Yuan Li,Zenghong Xie,Tingting Gao,Lin Li,Yongda Chen,Dan Xiao,Wen Liu,Baorong Zou,Biyu Lu,Xing Tian,Bo Han,Yaping Guo,Shaobao Zhang,Lin Lei,Mengxia Wang,Pei Li,Qiongfeng Liao
出处
期刊:Food & Function
[The Royal Society of Chemistry]
日期:2019-01-01
卷期号:10 (7): 4046-4061
被引量:45
摘要
Gallic acid (GA), a plant phenol found in fruits and vegetables, has been recently reported to attenuate ulcerative colitis (UC). However, the mechanism of GA in UC remains unknown. In this study, we investigated the therapeutic effects of GA on UC from the perspective of gut microbiota and supervised the metabolic alterations in vivo with 1H NMR-based metabolomics, which can provide a holistic view to understand the functions of GA in UC. Rats with dextra sulfate sodium (DSS)-induced colitis were rectally administrated with GA (6 mg kg-1) for 8 consecutive days. 16S gene sequencing was performed on feces samples to obtain bacterial community information. Urine and feces samples were analyzed with 1H NMR spectroscopy, and short chain fatty acids (SCFAs) in feces and colon contents were detected with gas chromatography. Our results showed that UC syndromes in the GA group were significantly attenuated. The microbial alterations in the DSS group were characterized by a decrease of probiotic bacteria, such as Lactobacillaceae and Prevotellaceae, and an increase of some pathogenic species, mainly in the Firmicutes and Proteobacteria phyla. GA treatment could modulate the microbiota composition towards a similar proportion to the control group. Metabolic data further revealed that the GA-induced metabolic changes focus on increasing carbohydrate metabolism (gluco-related metabolism) and bile acid (BA) metabolism and decreasing amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are by-products of interactions between the host and the microbiota. These findings demonstrate GA-induced alterations in metabolic and bacterial profiles in DSS-colitis, providing new insight into the attenuation of GA in UC.
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