医学
颈总动脉
大脑中动脉
冲程(发动机)
颈内动脉
闭塞
缺血
麻醉
颈外动脉
心脏病学
内科学
颈动脉
机械工程
工程类
作者
Luke R. Lemmerman,Hallie Harris,Maria Balch,Maria A. Rincon‐Benavides,Natalia Higuita‐Castro,David W Arnold,Daniel Gallego‐Perez
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2022-01-01
卷期号:12 (3)
被引量:2
标识
DOI:10.21769/bioprotoc.4305
摘要
Ischemic stroke is a leading cause of mortality and chronic disability worldwide, underscoring the need for reliable and accurate animal models to study this disease's pathology, molecular mechanisms of injury, and treatment approaches. As most clinical strokes occur in regions supplied by the middle cerebral artery (MCA), several experimental models have been developed to simulate an MCA occlusion (MCAO), including transcranial MCAO, micro- or macro-sphere embolism, thromboembolisation, photothrombosis, Endothelin-1 injection, and - the most common method for ischemic stroke induction in murine models - intraluminal MCAO. In the intraluminal MCAO model, the external carotid artery (ECA) is permanently ligated, after which a partially-coated monofilament is inserted and advanced proximally to the common carotid artery (CCA) bifurcation, before being introduced into the internal carotid artery (ICA). The coated tip of the monofilament is then advanced to the origin of the MCA and secured for the duration of occlusion. With respect to other MCAO models, this model offers enhanced reproducibility regarding infarct volume and cognitive/functional deficits, and does not require a craniotomy. Here, we provide a detailed protocol for the surgical induction of unilateral transient ischemic stroke in mice, using the intraluminal MCAO model. Graphic abstract: Overview of the intraluminal monofilament method for transient middle cerebral artery occlusion (MCAO) in mouse.
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