[Effects of activated CD4+ T cell-derived exosomes on cardiac remodeling after myocardial infarction].

OBJECTIVE: T cells in cardiac remodeling after myocardial infarction (MI). METHODS: T cells were incubated with cardiac fibroblasts (CFs) for 48 hours, and then the ability of CFs proliferation, migration and differentiation were detected by cell counting kit-8 (CCK-8) assay, Transwell assay, and immunofluorescence assay. (2) Experiment in vivo: 40 male C57 mice were divided into 4 groups according to random number table method, including control group (Ctrl group), sham operation group (Sham group), MI group, and exosome treatment group (MI+Exo group), with 10 in each group. The mice model of MI was established by ligating the left anterior descending coronary artery. In MI+Exo group, 40 μg/d exosomes were injected intravenously into the tail after modeling. Cardiac function and cardiac fibrosis post-MI were assessed by echocardiography and quantitative polymerase chain reaction (qPCR) at 4th week. RESULTS: ): 13.40±1.03 vs.4.96±0.36, all P < 0.05]. CONCLUSIONS: T cells promote cardiac remodeling following MI through transferring exosomes to CFs.