索拉非尼
细胞毒性
化学
肝细胞癌
药理学
白蛋白
药物输送
靶向给药
Zeta电位
结合
药品
MTT法
体外
医学
纳米颗粒
生物化学
癌症研究
材料科学
纳米技术
数学分析
数学
有机化学
作者
Ladan Dayani,Mohammad Dehghani,Mahmoud Aghaei,Somayeh Taymouri,Azade Taheri
标识
DOI:10.1016/j.jddst.2022.103142
摘要
Sorafenib (SF) is an anticancer drug used to treat hepatocellular carcinoma (HCC), but it is associated with a wide range of side effects. The objective of this research was to prepare SF-loaded albumin lipid nanoparticles (ALNs) consisting of medium-chain triglycerides and lactobionic acid-human serum albumin conjugate (LA-HSA conjugate) as a targeted drug delivery system of SF for the treatment of HCC. HSA was modified with ethylenediamine and conjugated to LA as a targeting agent. ALNs were characterized concerning particle size, zeta potential, drug encapsulation efficiency, and in vitro drug release. The cytotoxicity and cellular uptake of the targeted and untargeted ALNs were also evaluated in HepG2 cells. The results showed that the optimized targeted ALNs, which consisted of 10% (w/v) of HSA-LA, 5% (w/v) of poloxamer, and 5% of organic phase ratio with 0.1% (w/v) of lecithin, showed the particle size of 280.1 ± 4.4 nm, the zeta potential of −12.3 ± 0.9 mV, and the entrapment efficiency of 97.6 ± 2.2%. MTT assay and cellular uptake studies showed that the targeted ALNs had more cytotoxicity and cellular uptake than the untargeted ones in HepG2 cells (P < 0.05). Overall, the targeted delivery system reported here can enhance the therapeutic efficacy of SF in HCC.
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