化学
残留物(化学)
色谱法
电喷雾电离
蛋白质沉淀
肾
固相萃取
三级四极质谱仪
选择性反应监测
质谱法
串联质谱法
内分泌学
生物化学
生物
作者
Mariana Dolores-Hernández,Elvia Adriana Morales-Hipólito,Alma Villaseñor,Raquel López‐Arellano
出处
期刊:Food Chemistry
[Elsevier]
日期:2022-07-01
卷期号:382: 132287-132287
被引量:4
标识
DOI:10.1016/j.foodchem.2022.132287
摘要
Zilpaterol is a β-agonist compound which promotes fat loss and muscle gain in cattle, providing economic benefits. However, zilpaterol residues in the animal might introduce a significant risk to humans after consumption. In the present manuscript, a highly specific, sensitive method using Selected Reaction Monitoring (SRM) in positive electrospray ionization (ESI +) mode by liquid chromatography coupled to triple quadrupole mass spectrometry (LC-MS/MS) for plasma, muscle, liver and kidney is presented. For method development, composition of the aqueous mobile phase, precipitation agent, and solid phase extraction (SPE) conditions were optimized. The method was fully validated showing a good linearity and recovery average greater than or equal to 97 % for all matrices. The method was applied to residue depletion studies in cattle after withdrawal of zilpaterol supplementation at 3, 4, 5 and 6 days showing that tissues can be consumed by humans after 4th day of zilpaterol withdrawal.
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