Production of Monkey Antiserum against Mouse Growth Hormone

The antiserum against mouse growth hormone (MGH) was produced by immunizing monkeys and some basic immunological properties of the antiserum were studied.The antiserum formed single precipitin lines in agar gel double diffusion plate against both MGH preparation and the crude extract of mouse anterior pituitaries, but reacted with neither normal mouse serum nor the crude extracts of several viscera of mouse. Immunoelectrophoresis of the antiserum with MGH preparation showed a single precipitin arc at the corresponding site of MGH. The precipitin lines between MGH preparation and monkey antiserum and rabbit antiserum to MGH produced previously by us fused completely. Furthermore, the biological activity of MGH was significantly inhibited by the antiserum. All the results have demonstrated that the antiserum obtained in the present experiment contains the antibody specific only to MGH.The cross-reaction test of the antiserum indicates that MGH shares immunologically reactive site identical to that contained in growth hormones of rats, rabbits, goats and cows.