丹参
安普克
SH-SY5Y型
蛋白激酶B
活性氧
氧化应激
化学
PI3K/AKT/mTOR通路
神经保护
蛋白激酶A
神经母细胞瘤
三磷酸腺苷
激酶
细胞生物学
生物化学
药理学
细胞凋亡
生物
医学
细胞培养
中医药
替代医学
病理
遗传学
作者
Hengai Zhang,Mei Gao,Li Zhang,Yan Zhao,Lili Shi,Bai-Nian Chen,Yuehua Wang,Shoubao Wang,Guanhua Du
标识
DOI:10.1016/j.bbrc.2012.04.021
摘要
Salvianolic acid A (Sal A) is a polyphenol extracted from the root of the Salvia miltiorrhiza bunge. Hydrogen peroxide (H(2)O(2)) is a major reactive oxygen species (ROS), which has been implicated in stroke and other neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease. In this study, we investigated the neuroprotective effects of Sal A in human SH-SY5Y neuroblastoma cells against H(2)O(2)-induced injury. Our results showed that cells pretreated with Sal A exhibited enhanced neuronal survival and that this protection was associated with an increase in adenosine triphosphate (ATP) and the stabilization of mitochondrial membrane potential. In addition, Sal A markedly decreased the excessive activation AMP-activated protein kinase (AMPK) and the serine-threonine protein kinase, Akt, in SH-SY5Ycells induced by H(2)O(2). In conclusion, our results demonstrated that Sal A protects SH-SY5Y cells against H(2)O(2)-induced oxidative stress and these protective effects are related to stress tolerance and not energy depletion via inhibition of the AMPK and Akt signaling pathway.
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