Genipin is the iridoid glycoside geniposide isolated from the fruit of Gardenia jasminoides Ellis. UV, TLC method was used to investigate the reactivity of genipin with leather fibers and their reaction theory. The tanning property of genipin, as well as the tanning conditions such as dosage of genipin, time, temperature and pH value etc was analyzed by DSC. The results show that the Tp of DSC curve reachs 85°C and the hide powder becomes dark blue color under the following condition: dosage of ginipin is 5% of dried hide powder, pH value is 7·5, tanning time is 24h. Therefore genipin could be used as a potential, regenerative, cleansing and effective tanning agent and dyestuff.