荧光
化学
配体结合分析
配体(生物化学)
组合化学
G蛋白偶联受体
生物测定
竞争性约束
生物物理学
受体
生物化学
纳米技术
计算生物学
生物
材料科学
量子力学
物理
遗传学
作者
Yilei Yang,Qinghao Zhang,Mei Hua Gao,Xiaohong Yang,Ziwei Huang,Jing An
出处
期刊:Biochemistry
[American Chemical Society]
日期:2014-07-28
卷期号:53 (30): 4881-4883
被引量:8
摘要
We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, with a capability comparable to that seen with a conventional CXCR4 antibody. This nonradioactive, direct, and CXCR4-specific high-affinity screening system provides a new platform for CXCR4-targeted drug screening, as well as for the development of new probes for other GPCRs.
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