防御素
异源的
异源表达
生物
计算生物学
抗菌肽
大肠杆菌
生物技术
微生物学
生物化学
抗菌剂
重组DNA
基因
作者
Luiz Rodrigo Saldanha Gazzaneo,Valesca Pandolfi,A. L. S. Jesus,Sergio Crovella,Ana Maria Benko-Iseppon,Antonio Carlos de Freitas
出处
期刊:Current Protein & Peptide Science
[Bentham Science]
日期:2017-02-06
卷期号:18 (4): 391-399
被引量:4
标识
DOI:10.2174/1389203717666160625070414
摘要
Defensins are a superfamily of antimicrobial peptides, present in vertebrates, invertebrates, fungi and plants, suggesting that they appeared prior to the divergence in eukaryotes. The destitution of toxicity to mammalian cells of plant defensins has led to a new research ground, i.e., their potential medical use against human infectious diseases. Isolating defensins from natural sources, like plant tissues, can be time-consuming, labor intensive and usually present low yields. Strategies for large-scale production of purified active defensins have been employed using heterologous expression systems (HES) for defensin production, usually based in E. coli system. Like any other technology, HES present limitations and drawbacks demanding a careful experimental design prior the system selection. This review is proposed to discuss some of the major concerns when choosing to heterologously express plant defensins, with special attention on bacterial expression systems. Keywords: E. coli, fusion, carrier protein, secretion signal, inclusion bodies, antimicrobial peptides.
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