We compared bumblebee pollination and natural-pollination on strawberry in greenhouse. The results showed that the average yield of strawberry was 190.45 kg/hm~2, increased 38.81% than natural-pollination, and the average fruit weight was 15.10 g with bumblebee pollination, increased 37.02% than natural-pollination with significant difference.Fruit malformation rate of strawberry reduced 81.82% treated with bumblebee pollination, while dissolubility sugar of strawberry fruittreated with bumblebee pollination andnatural pollination was 11.82% and 10.85% seperately, without significant difference; strawberry treated with bumblebee pollination which were coloring consistence with bright color,sweet and delicious, and were better than natural-pollination, and the output yield increased 48.73%.