The regional cooperation of the Yangtze River Delta is one of the earliest in China,and Shanghai Expo 2010 accelerates the process of Yangtze River Delta integration.This paper reviews the basis of the basic course of Yangtze River Delta regional cooperation before Shanghai Expo,summarizes the new progress of Yangtze River Delta institutional cooperation during the time of preparing for and hosting Shanghai Expo,analyzes the problems of the Yangtze River Delta regional cooperation,puts forward the development path and countermeasures to strengthen the Yangtze River Delta integration institutional cooperation with the Expo' s effects.