色谱法
化学
高效液相色谱法
没食子酸
儿茶素
质谱法
梯度洗脱
分辨率(逻辑)
咖啡因
定量分析(化学)
多酚
生物化学
人工智能
内分泌学
医学
抗氧化剂
计算机科学
作者
Monica Araya-Farias,Alain Gaudreau,Élodie Rozoy,Laurent Bazinet
摘要
An effective and rapid HPLC-MS method for the simultaneous separation of the eight most abundant tea catechins, gallic acid, and caffeine was developed. These compounds were rapidly separated within 9 min by a linear gradient elution using a Zorbax SB-C18 packed with sub 2 μm particles. This methodology did not require preparative and semipreparative HPLC steps. In fact, diluted tea samples can be easily analyzed using HPLC-MS as described in this study. The use of mass spectrometry detection for quantification of catechins ensured a higher specificity of the method. The percent relative standard deviation was generally lower than 4 and 7% for most of the compounds tested in tea drinks and tea extracts, respectively. Furthermore, the method provided excellent resolution for folate determination alone or in combination with catechins. To date, no HPLC method able to discriminate catechins and folates in a quick analysis has been reported in the literature.
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