Evaluation of cold-heat characteristics of Pulsatillae Radix

Objective To evaluate the cold-heat characteristics of Pulsatillae Radix by the method we have set up.Methods MTT assay was used to investigate the effect of Pulsatillae Radix on the proliferation of HeLa and SGC-7901 cells in vitro.The morphological changes of HeLa and SGC-7901 cells treated with Pulsatillae Radix were observed through inverted microscope.Trypan-blue staining was used to analyze the cytotoxicity of Pulsatillae Radix.Results Pulsatillae Radix could inhibit the proliferation of HeLa and SGC-7901 cells in the concentration range of 10—400 μg/mL.Morphological observation showed Pulsatillae Radix in the concentration range we have chosen could decrease the density of HeLa and SGC-7901 cells and condense the cells.Trypan-blue staining showed Pulsatillae Radix had no cytotoxicity on HeLa and SGC-7901 cells.Conclusion Pulsatillae Radix is a cold drug and has no cytotoxicity.