Objective To investigate the value of two-dimensional and three-dimensional ultrasound in the diagnosis of fetal cheiloschisis.Methods Two-dimensional ultrasound was used to observe nasolabial development,then three-dimensional ultrasound was applied to take dynamic facial imaging in out-patient women with pregnancy of 18-40 weeks.Results For 18-24 weeks fetal cheiloschisis,images from three-dimensional ultrasound were clear and visual,while the results of two-dimensional ultrasound were time-consuming and operator-dependent.For 36-40 weeks fetal cheiloschisis,images from three-dimensional ultrasound were hard to obtain,while two-dimensional ultrasound could take facial imaging through nonstandard section.Both three-dimensional ultrasound and two-dimensional ultrasound took images of 24-36 weeks fetus effectively.Conclusion Both two-dimensional ultrasound and three-dimensional ultrasound diagnose fetal cheiloschisis effeciently.Two-dimensional ultrasound and three-dimensional ultrasound have advantages during different pregnanct periods.Three-dimensional ultrasound shows obvious advantages in the diagnosis of fetal cheiloschisis in early pregnancy,while the two-dimensional ultrasound shows a little advantage in the diagnosis of fetal cheiloschisis in late pregnancy.