Establishment of a multiplex genotyping method for single nucleotide polymorphisms of genes associated with coronary artery disease

Objective To establish a multiplex genotyping method for single nucleotide polymorphisms(SNPs) of genes associated with coronary artery disease(CAD).Methods DNA samples were extracted from peripheral white blood cells and 44 tag SNPs of genes associated with CAD for Chinese Han nationality were selected in the current work.Three primers were designed for each SNP.After polymerase chain reaction,extension and hybridization,genotypes of SNPs were investigated with the Genome Lab SNP stream genotyping system.Results Thirty two SNPs were genotyped and the rate of succeed SNP genotyping was 72.7%(32/44).The average genotyped rate of the 32 SNPs was 98.3% in the test of 583 DNA samples.Conclusion Thirty two plexs SNPs genotyping methods established in the current work were quick,exact,high-throughput,easy operation and microanalyse.