Recent Advances in Yeast Cell-Surface Display Technology

Yeast cell has a perfect system for protein post-translational modification and secretion.The cell surface display technology based on yeast is a novel eukaryotic protein display technology.It has been successfully used for protein recognition,immobilization and remediation,and becomes an important tool for the study of protein engineering.Yeast cell surface display technology is divided into two systems,which are agglutinin and flocculin,according to the different insertion sites of the yeast cell wall for target heterologous proteins.If introduced to animal nutrition research,this technology will be a promising strategy to reduce the methane production by the regulation on ruminal microbial ecology,which is inducing of production of antibodies for protozoa and methanogen in host;highly active whole cell biocatalysts will be produced by increasing fiber utilization in ruminant and monogastric animal.Therefore,mechanism,characteristic and application of the technology are reviewed in this paper.