Expression of aquaporin-5 in submandibular glands of rat with Sjogren's syndrome

Objective To investigate the influence of Astragalus Danshen compound on expression distribution of aquaporin-5(AQP5) in submandibular salivary glands of Sjogren′s syndrome(SS) on rats.Methods Submandibular gland tissue was extracted from Wistar rats before blending and emulsification with complete Freund′s adjuvant(CFA).After that,subcutaneous injection of DTP vaccine was conducted on the back to enhance immunity.The expression distribution of aquaporin-5(AQP5) in submandibular salivary glands of Sjogren′s syndrome(SS) rats was indicated by Western blot analysis.Results After immunization for 7 weeks of SS rats,pathological changes were produced similar to the salivary gland in human Sjogren′s syndrome.It suggested that a large number of lymphocyte cells showed different invasive depths and effective immunization.Western blot analysis showed that there was a 42 KD in each group.The expressed numbers of the control group were normal(1.35±0.13),but that of the normal saline group was obviously abnormal(1.03±0.08).There were clear enhancements of expression numbers on the Astragalus Danshen compound of high-dose(1.31±0.15).Compared with the control group,the expression of AQP5 in the normal saline group of rat model significantly reduced(P0.05).Astragalus Danshen compound of high-dose significantly increased the expression of AQP5(P0.05).Conclusion Astragalus Danshen compound enhanced the release of water molecules in channel of submandibular gland,by floating the expression of AQP5,so as to improve the secretory function of salivary glands and release the thirsty symptoms of Sjogren′s syndrome.