群体感应
铜绿假单胞菌
毒力
微生物学
基因
抗生素耐药性
机制(生物学)
计算生物学
生物
抗生素
细菌
遗传学
认识论
哲学
作者
Weijie Sun,Yuan Wang,Liuhong Shen,Kangmin Duan
出处
期刊:PubMed
日期:2009-08-01
卷期号:25 (8): 1173-9
摘要
Quorum sensing is an important gene regulatory mechanism in Pseudomonas aeruginosa and controls the expression of numerous virulence factors. We designed and constructed a screening system for quorum-sensing inhibitors. We developed the system by using the lasI and rhlA promoters fused with promoterless sacB as reporters. Using this system we screened a number of Chinese herb extracts, and identified three herb extracts containing inhibitors to the quorum-sensing system and to its regulated genes. The screening system developed was highly efficient and sensitive. It could serve as a useful tool to identify herb compounds that block infections but unlikely render antibiotic resistance in pathogens.
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