核糖体
蛋白质生物合成
体外
生物
生物化学
基因
DNA
核糖核酸
计算生物学
化学
分子生物学
作者
Hani Zaher,Rachel Green
出处
期刊:Methods in Enzymology
日期:2014-01-01
卷期号:: 3-15
被引量:4
标识
DOI:10.1016/b978-0-12-420120-0.00001-3
摘要
This protocol describes the methods used to generate protein in a cell-free system derived from E. coli. The in vitro synthesis of protein has been used in studying many ribosome-based gene regulation steps (Gong and Yanofsky, 2001). Such techniques have also been used to study protein-protein, protein-DNA, and protein-RNA interactions, and to produce radiolabeled protein species. More recently, such approaches have been utilized to produce large quantities of toxic proteins for proteomic and structural studies (Yokoyama et al., 2000).
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