[The conditions of Helicobacter pylori colonization in different gastric diseases].

To investigate the condition of Helicobacter pylori (Hp) colonization in different gastric diseases and the application of immunostaining technique in Hp density measurement.Biopsy specimens of gastric mucosa were obtained from 174 patients with different gastric diseases confirmed by pathological examination, 99 males and 75 females, aged 53.37 (19-87), 135 from Zhuanghe, a high risk area of gastric cancer, and 39 from Shenyang, a low risk area. Hematoxylin and eosin (HE) staining and immunohistochemistry were used to detect the Hp density in different parts of gastric mucosa.When the grade of Hp density in the gastric mucosa epithelium cell surface and lumen of gland < 1 there was a significant difference between HE stain and immunohistochemistry (chi(2) = 9.446, P < 0.01), however, when the grade of Hp density in the gastric mucosa epithelium cell surface and lumen of gland > 1 there was no significant difference between them (P > 0.05). The grade of Hp density of gastric mucosa epithelium cell surface and lumen of gland in superficial gastritis was > 1, significantly higher than those of atrophic gastritis group (23.8%) and gastric cancer group (16.7%) (chi(2) = 2.780, P > 0.05; chi(2) = 4.876, P > 0.05). The Hp colonization density in the gastric mucosa epithelium cell surface and lumen of the patients from Zhuanghe region was significantly lower than that of the patients from Shenyang region (chi(2) = 5.915, P = 0.015); and the Hp density in the gastric mucus in the patients from Zhuanghe was significantly higher than that of the patients from Shenyang region (chi(2) = 8.879, P = 0.003, chi(2) = 22.036, P = 0.000). The grades of Hp density within the surface mucous gel layer in atrophic gastritis and gastric cancer was significantly higher than that of the superficial gastritis group (53.7%, chi(2) = 11.002, P < 0.05; chi(2) = 19.401, P < 0.05).The density of Hp colonization in gastric mucosa epithelium cell surface and lumen of gland gradually decreases in the order of superficial gastritis-atrophic gastritis-gastric cancer, while the density of Hp within the surface mucous gel layer increases in the order of superficial gastritis- atrophic gastritis-gastric cancer.