生产力
代谢组学
自噬
酵母
生物化学
化学
生物技术
生物
色谱法
细胞凋亡
经济
宏观经济学
作者
Sven Dierickx,Maximilien Souvereyns,Sophie L.K.W. Roelants,Marilyn De Graeve,Lieven Van Meulebroek,Sofie L. De Maeseneire,Wim K.G. Soetaert,Lynn Vanhaecke
标识
DOI:10.1016/j.nbt.2023.02.002
摘要
Sophorolipids are biobased and biodegradable glycolipid surface-active agents contributing to the shift from petroleum to biobased surfactants, associated with clear environmental benefits. However, their production cost is currently too high to allow commercialisation. Therefore, a continuous sophorolipid production process was evaluated, i.e., a retentostat with an external filtration unit. Despite an initial increase in volumetric productivity, productivity eventually declined to almost 0gL-1 h-1. Following comprehensive metabolomics on supernatant obtained from a standardised retentostat, we hypothesised exhaustion of the N-starvation-induced autophagy as the main mechanism responsible for the decline in bolaform sophorolipid productivity. Thirty-six metabolites that correlate with RNA/protein autophagy and high sophorolipid productivity were putatively identified. In conclusion, our results unveil a plausible cause of this bola sophorolipid productivity decline in an industrially relevant bioreactor set-up, which may thus impact majorly on future yeast biosurfactant regulation studies and the finetuning of bola sophorolipid production processes.
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