补骨脂素
肝损伤
胆汁酸
胆固醇7α羟化酶
流出
免疫印迹
CYP27A1
化学
新陈代谢
内分泌学
内科学
运输机
肝肠循环
药理学
生物化学
生物
医学
基因
DNA
作者
Meng‐ying Chen,Qin Wang,Zhao‐jun Meng,Wei‐jie Men,Juyang Huang,Bin Yu,Kun Zhou
摘要
Abstract Psoralen is a major component of Fructus Psoraleae that could induce liver injury. In this study, C57BL/6J mice were administered with psoralen at doses of 80 mg/kg for 3, 7 and 14 days. Blood and liver samples were collected for serum biochemistry and histopathology examinations, respectively. Psoralen led to liver injury with significantly increased liver weight and liver coefficient and up regulated serum ALT, AST and TG but down regulated serum TC and TP. The expression of bile acid‐associated transporters and enzymes was detected by western blot, and the results showed that psoralen significantly down‐regulates the expressions of CYP7A1, CYP27A1, BSEP and OSTα protein while up‐regulates the expressions of HMGCR and FASN, resulting in the obstacles of bile acid efflux in the liver. The contents of 24 kinds of bile acids in the liver were measured by LC–MS/MS, and the results showed that psoralen led to the accumulation of unconjugated bile acids in the liver, such as ALCA and CA, which were more severe in male mice than female mice. It was indicated that psoralen may disrupt the balance of bile acid metabolism by inhibiting the expression of the efflux transporter, which then leads to liver damage.
科研通智能强力驱动
Strongly Powered by AbleSci AI