[MDC_Alternative_2D_hiPSC-CM_diff] hiPSC Cardiomyocyte differentiation v2

This protocol outlines asmall molecules based, 2D differentiation methodfor generating high-purity human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Optimized for reproducibility across hiPSC lines, the procedure achieves robust cardiac differentiation through sequential modulation of Wnt signaling, yielding functional beating cardiomyocytes by days 7–10.