生物
突变体
稻黄单胞菌
水稻
水稻黄单胞菌。稻瘟
拟南芥
过敏反应
细胞生物学
几丁质酶
拟南芥
先天免疫系统
调解人
免疫
蛋白质亚单位
微生物学
基因
遗传学
免疫系统
植物抗病性
作者
Peng Zhang,Xiaoding Ma,Lina Liu,Chanjuan Mao,Yongkang Hu,Bingxiao Yan,Jia Guo,Xinyu Liu,Jinxia Shi,GangSeob Lee,Xi Pan,Yiwen Deng,Zhengguang Zhang,Zhensheng Kang,Yongli Qiao
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2023-02-23
卷期号:192 (2): 1132-1150
被引量:5
标识
DOI:10.1093/plphys/kiad120
摘要
Abstract Lesion mimic mutants (LMMs) are valuable genetic resources for unraveling plant defense responses including programmed cell death. Here, we identified a rice (Oryza sativa) LMM, spotted leaf 38 (spl38), and demonstrated that spl38 is essential for the formation of hypersensitive response-like lesions and innate immunity. Map-based cloning revealed that SPL38 encodes MEDIATOR SUBUNIT 16 (OsMED16). The spl38 mutant showed enhanced resistance to rice pathogens Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae (Xoo) and exhibited delayed flowering, while OsMED16-overexpressing plants showed increased rice susceptibility to M. oryzae. The OsMED16-edited rice lines were phenotypically similar to the spl38 mutant but were extremely weak, exhibited growth retardation, and eventually died. The C-terminus of OsMED16 showed interaction with the positive immune regulator PATHOGENESIS RELATED 3 (OsPR3), resulting in the competitive repression of its chitinase and chitin-binding activities. Furthermore, the ospr3 osmed16 double mutants did not exhibit the lesion mimic phenotype of the spl38 mutant. Strikingly, OsMED16 exhibited an opposite function in plant defense relative to that of Arabidopsis (Arabidopsis thaliana) AtMED16, most likely because of 2 amino acid substitutions between the monocot and dicot MED16s tested. Collectively, our findings suggest that OsMED16 negatively regulates cell death and immunity in rice, probably via the OsPR3-mediated chitin signaling pathway.
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