银纳米粒子
纤维素
细菌纤维素
纳米纤维素
生物材料
抗菌剂
纳米技术
化学
生物高聚物
材料科学
纳米颗粒
化学工程
有机化学
聚合物
工程类
作者
Cassandra Scott,Nick-Hugh Wisdom,Kevin Coulter,Sylvie Bardin,Janice L. Strap,Liliana N. Trevani
标识
DOI:10.1021/acs.jchemed.2c00712
摘要
This laboratory exercise integrates chemistry and biology concepts to give third/fourth-year undergraduate students an opportunity to apply knowledge from different subject areas to address a real-world biomedical issue such as pathogen inhibition using composite materials. It involves the preparation of a bacteria-derived cellulosic biopolymer through microbial cultivation, impregnation of the bacterial cellulose (BC) with silver nanoparticles (AgNPs), followed by the analysis of the materials and the antimicrobial properties of the biomaterial-AgNPs composites. The methods are relatively simple and use inexpensive chemicals. A Tollens type approach is adopted to produce silver nanoparticles-bacterial cellulose (AgNPs-BC) composites by the reduction of [Ag(NH3)2]+ complex embedded in the cellulose matrix. The samples were dried by two different methods: freeze-drying or vacuum-drying. The dried AgNPs-BC films were evaluated for antimicrobial properties against a test organism, in this example, Pseudomonas aeruginosa, a Gram-negative biosafety containment level 2 (BSL 2) bacterium, using an agar diffusion test. For additional flexibility and customization, options for dividing the chemistry/biology content of this laboratory into smaller units with an emphasis on characterization techniques of nanomaterials for chemistry majors are also discussed.
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