甲壳素
化学
衍生化
分馏
乙酰化
色谱法
学位(音乐)
食品科学
壳聚糖
高效液相色谱法
生物化学
声学
基因
物理
作者
Elisabeth Dhondt,Lise Soetemans,Leen Bastiaens,Miranda Maesen,Vincent Jespers,Bert Van den Bosch,Stefan Voorspoels,Kathy Elst
标识
DOI:10.1016/j.carres.2019.107899
摘要
Insects are considered a promising alternative protein source for food and feed, but contain significant amounts of chitin, often undesirable due to indigestibility, disagreeable texture and negative effect on nutrients intake. Fractionation strategies are thus increasingly being applied to isolate and valorize chitin separately. The analysis of chitin generally requires an intensive pretreatment to remove impurities, and derivatization to generate sufficient detector response. In this work, a liquid chromatography method, without pretreatment nor derivatization, was developed for the simultaneous determination of chitin content and degree of acetylation in non-purified samples of black soldier fly (BSF) larvae. The method is found to be more suitable, compared to traditional methods, for assessing high degrees of acetylation. For the first time, the degree of acetylation of BSF chitin (81 ± 2%) is reported. Additionally, the chitin content of BSF soft tissues is estimated at approximately 20% of the total chitin content (8.5 ± 0.1%).
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