肠上皮
转录组
单元格排序
人口
计算生物学
电池类型
细胞
细胞生物学
上皮
基因表达
生物
单细胞分析
遗传学
基因
医学
环境卫生
作者
Claudia Capdevila,Ruben I. Calderon,Erin C. Bush,Kismet Sheldon-Collins,Peter A. Sims,Kelley S. Yan
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 129-153
被引量:6
标识
DOI:10.1007/978-1-0716-0747-3_8
摘要
Emerging single-cell technologies, like single-cell RNA sequencing (scRNA-seq), enable the study of heterogeneous biological systems at cellular resolution. By profiling the set of expressed transcripts in each cell, single-cell transcriptomics has allowed for the cataloging of the cellular constituents of multiple organs and tissues, both in health and disease. In addition, these technologies have provided mechanistic insights into cellular function, cell state transitions, developmental trajectories and lineage relationships, as well as helped to dissect complex, population-level responses to environmental perturbations. scRNA-seq is particularly useful for characterizing the intestinal epithelium because it is a dynamic, rapidly self-renewing tissue comprised of more than a dozen specialized cell types. Here we discuss the fundamentals of single-cell transcriptomics of the murine small intestinal epithelium. We review the principles of proper experimental design and provide methods for the dissociation of the small intestinal epithelium into single cells followed by fluorescence-activated cell sorting (FACS) and for scRNA-seq using the 10× Genomics Chromium platform.
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