This study was subjected to investigate antimicrobial (antibacterial and antifungal) and antioxidant properties of Salmacis virgulata methanolic extract. The antioxidant property of the extract was assessed by 2, 2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Disc diffusion techniques were used to determine the antibacterial and antifungal activity of the extract. DPPH free radical scavenging effect of the extract was compared with standard antioxidant ascorbic acid and its show significant result. Salmacis virgulata (77.51%) showed potent activity at the concentration of 100µg/ml than compared to standard ascorbic acid. This study supports, methanolic extract of Salmacis virgulata has potential antimicrobial and antioxidant activity.